Dottie is the love interest of Pee-wee Herman from the 1985 film, Pee-wee's Big Adventure.
She is played by EG Daily who would later voice Tommy Pickles from Rugrats and Rudy Tabootie from ChalkZone.
He first sees her while Pee-wee is at a bicycle shop called "Chuck's Bike-O-Rama" before he discovers that his bike has gone missing. After Pee-wee realizes that the bike was not stolen by Francis Buxton and Pee-wee refuses to let Dottie help him find his bike, Pee-wee goes to a fortune teller's shop to discover that the bike is far away and hidden in the basement of the Alamo and Pee-wee tries to find the bicycle by hitch-hiking to San Antonio and discovers that the Alamo doesn't have a basement. He calls Dottie on the phone to apologize for what he have said earlier. After a dance to Tequila and a crash through an advertisement sign and going to the hospital. Pee-wee discovers that his bike is at Warner Bros. Studios in Burbank, California and decides to disguise himself as a nun to get his bike back and Pee-wee ends up in a wild chase to the burning pet shop before the police can arrest him and Pee-wee tries to explain everything to the head of the Warner Bros. Studios, but Warner Bros think Pee-wee's story is excellent and great as Dottie arrives to give Pee-wee his bike back and the two reconcile. At the drive-in theater, Pee-wee and Dottie began dating after selling all the snacks to everybody from his adventure and is reunited with his bike. By the end of the film, she and Pee-wee rode off in their bikes as the film comes to an end.