Penelope Pussycat (voiced by Victoria Jackson) served as another love interest for Garfield. She appeared in Garfield and Friends, quickly becoming a regular (with three episodes being labeled as "Penelope Episodes"). She lives in an Italian restaurant, which is no doubt the main reason that Garfield goes out with her. The fact that Garfield enjoys eating more than being with her annoys Penelope, but she willingly goes out with him anyway, because as she says, "I don't care what we do as long as we do it together."
Being substitution of Arlene in "Garfield and Friends", Penelope has similar bodyshape like Arlene with exception of her characteristic hair, beauty spot on cheek, blue eyes and smaller lips ( light pink in "Perils of Penelope" and "The Genuine Article", darker in the following episodes ). Apart from that, Penny has opposite personality to Arlene - she barely cracks jokes at Garfield's expense ( least less frequent than Arlene does ) and her relationship looks more like one-side attraction. In fact, Garfield appreciates Penelope rather for her living in an Italian Restaurant, which can be seen in "The Third Penelope Episode".
- Penelope tends to walk on all four of her legs instead of two.
- She has a big family including her unnamed mother, her brother Al, his unnamed wife with their three children, a deceased father, grandparents, an aunt, an uncle and many cousins.
- Her design was changed during the episodes - originally she had beige fur colour ("The Perils of Penelope" and "The Genuine Article"), replaced with gray in "The Second Penelope Episode", "The Garfield Musical", "The Third Penelope Episode" , "The Garfield Rap" and "The Guy of Her Dreams".